Rules for All Projects


Regeneron ISEF Ethics Statement
Regeneron ISEF Eligibility/Limitations
Regeneron ISEF Requirements
Continuation of Projects
Team Projects

The International Rules and Guidelines for Science Fairs is
available at in multiple formats.
Familiarity with the rules is critical for students, parents, teachers,
mentors, fair directors and local and affiliated fair Scientific
Review Committees (SRC) and Institutional Review Boards (IRB).

Ethics Statement

Student researchers, as well as adults who have a role in their projects, are expected to maintain the highest ethical standards. These include, but are not limited to:

  • Integrity. Honesty, objectivity, and avoidance of conflicts of interest are expected during every phase of the research. The project should reflect independent research done by the student(s), and represent only one year’s work.
  • Legality. Compliance with all federal, state and local laws and regulations is essential. In addition, projects conducted outside the U.S. must also adhere to the laws of the country and jurisdiction in which the project was performed. All projects must be approved by a Scientific Review Committee (SRC), and when necessary must also be approved by an Institutional Review Board (IRB), Institutional Animal Care and Use Committee (IACUC), and/or Institutional Biosafety Committee (IBC).
  • Respect for Confidentiality and Intellectual Property. Confidential communications, as well as patents, copyrights, and other forms of intellectual property must be honored. Unpublished data, methods, or results may not be used without permission, and credit must be given to all contributions to research.
  • Stewardship of the Environment. It is the responsibility of the researcher and the adults involved to protect the environment from harm. Introduction or disposal of non-native, genetically-altered, and/or invasive species, (e.g. insects, plants, invertebrates, vertebrates), pathogens, toxic chemicals or foreign substances into the environment is prohibited. It is recommended that students reference their local, state or national laws and regulations and quarantine lists, including if considering using “catch and release” fishing procedures.
  • Animal Care. Proper care and respect must be given to vertebrate animals. The guiding principles for the use of animals in research includes the following “Four R’s”: Replace, Reduce, Refine, Respect.
  • Human Participant Protection. The highest priority is the health and well-being of the student researcher(s) and human participants.
  • Potentially Hazardous Biological Agents (PHBAs). It is the responsibility of the student and adults involved in the project to conduct and document a risk assessment, and to safely handle and dispose of organisms and materials.

Scientific fraud and misconduct are not condoned at any level of research or competition. This includes plagiarism, forgery, use or presentation of other researcher’s work as one’s own and fabrication of data. Fraudulent projects will fail to qualify for competition in affiliated fairs and Regeneron ISEF. Society for Science and the Public reserves the right to revoke recognition of a project subsequently found to have been fraudulent.


1. Each Regeneron ISEF-affiliated fair may send to Regeneron ISEF the number of projects provided by their affiliation agreement.

2. A student must be selected by an Regeneron ISEF-affiliated fair, and meet both of the following:

  • a. be in grades 9-12 or equivalent; and
  • b. not have reached age 20 on or before May 1 preceding the Regeneron ISEF.

3. English is the official language of the Regeneron ISEF. Student project boards and abstracts must be in English.

4. Each student is only allowed to enter one project. That project may include no more than 12 months of continuous research and may not include research performed before January 2023.

5. Team projects must have no more than three members. Teams competing at Regeneron ISEF must be composed of members who all meet Regeneron ISEF eligibility.

6. Students may compete in only one Regeneron ISEF affiliated fair, except when proceeding to a state/national fair affiliated with the Regeneron ISEF from an affiliated regional fair.

7. Projects that are demonstrations, ‘library’ research or informational projects, and/or ‘explanation’ models or kit building are not appropriate for the Regeneron ISEF.

8. All sciences and engineering disciplines are represented at ISEF and projects compete in one of the 21 categories. Review a complete list of categories and sub-categories with definitions.

9. A research project may be a part of a larger study performed by professional scientists, but the project presented by the student must be only their own portion of the complete study.



1. All domestic and international students competing in an Regeneron ISEF-affiliated fair must adhere to all rules as set forth in this document.

2. All projects must adhere to the Ethics Statement above.

3. It is the responsibility of the student and the Adult Sponsor to evaluate the study to determine if the research will require forms and/or review and approval prior to experimentation.

4. Projects must adhere to local, state and U.S. Federal laws, regulations and permitting conditions. In addition, projects conducted outside the U.S. must also adhere to the laws of the country and jurisdiction in which the project was performed.

5. The use of non-animal research methods and alternatives to animal research are strongly encouraged and must be explored before conducting a vertebrate animal project.

6. Introduction or disposal of non-native, genetically-altered, and/or invasive species (e.g. insects, plants, invertebrates, vertebrates), pathogens, toxic chemicals or foreign substances into the environment is prohibited. It is recommended that students reference their local, state or national regulations and quarantine lists.

7. Projects competing at Regeneron ISEF must have an exhibit that adheres to Regeneron ISEF Display & Safety requirements and is visible during all operable hours of the exhibit hall without reliance on electricity or internet connections.

8. All projects must adhere to the requirements of the affiliated fair(s) in which it competes to qualify for participation in Regeneron ISEF. Affiliated fairs may have additional restrictions or requirements. Knowledge of these requirements is the responsibility of the student and Adult Sponsor.

Approval and Documentation

1. Project documentation should begin before experimentation with the current forms available. Projects involving human participants, vertebrate animals, and potentially hazardous biological agents must be reviewed and approved by a local or regional Institutional Review Board (IRB) or Scientific Review Committee (SRC) prior to the start of experimentation. At the start of the project, which may in some cases be prior to experimentation begins, a local or regional Institutional Review Board (IRB) or Scientific Review Committee (SRC) with the ISEF-affiliated fair must review and approve most projects involving human participants, vertebrate animals, and potentially hazardous biological agents. Note: If a project involves the testing of a student designed invention, prototype or concept by a human, an IRB review and approval may be required prior to experimentation. See Human Participants Rules for details.

2. Every student must complete the Student Checklist (1A), a Research Plan/Project Summary and Approval Form (1B) and review the project with the Adult Sponsor in coordination with completion by the Adult Sponsor of the Checklist for Adult Sponsor (1).

3. A Qualified Scientist is required for all studies involving Biosafety Lab-2 (BSL-2) potentially hazardous biological agents and DEA-controlled substances and is also required for many human participant studies and many vertebrate animal studies.

4. After initial IRB/SRC approval (if required), any proposed changes in the Student Checklist (1A) and Research Plan/Project Summary must be re-approved before laboratory experimentation/data collection resumes.

5. Projects which are continuations of a previous year’s work and which require IRB/SRC approval must undergo the review process with the current year ResearchPlan/Project Summary prior to experimentation/data collection for the current year.

6. Any continuing project must document that the additional research is new and different. (Continuation/Research Progression Projects Form (7).

7. If work was conducted in a regulated research institution, industrial setting or any work site other than home, school or field at any time during the current Regeneron ISEF project year, the Regulated Research Institutional/Industrial Setting Form (1C) must be completed and displayed at the project booth.

8. After experimentation, each student or team must submit a (maximum) 250-word, one-page abstract which summarizes the current year’s work. The abstract must describe research conducted by the student, not by the supervising adult(s).

9. A project data book and research paper are not required, but are strongly recommended for judging purposes. Regional or local fairs may require a project data book and/or a research paper.

10. All signed forms, certifications, and permits must be available for review by all regional, state, national and international affiliated fair SRCs in which the student(s) participate. This review must occur after experimentation and before competition.

Digital Paperwork and Signatures

Submission of forms generated by a digital system are allowable under the following conditions:

1. The forms must have the same content and order as the Regeneron ISEF forms.

2. Digital signatures must have a verification system via login and have a time and date stamp to indicate this authentication.

3. Paperwork submitted to Society for Science & the Public for Regeneron ISEF must be scanned and submitted via the online portal.

Continuation/ Research Progression of Projects

1. As in the professional world, research projects may build on work performed previously. A valid continuation project is a sound scientific endeavor. Students will be judged only on laboratory experiment/data collection performed over 12 continuous months beginning no earlier than January 2023 and ending May 2024.

2. Any project based on the student’s prior research could be considered a continuation/research progression project. These projects must document that the additional research is a substantive expansion from prior work (e.g. testing a new variable or new line of investigation). Repetition of previous experimentation with the same methodology and research question, even with an increased sample size, is an example of an unacceptable continuation.

3. The display board and abstract must reflect the current year’s work only. The project title displayed in the finalist’s booth may mention years (for example, “Year Two of an Ongoing Study”). Previous year’s databooks, research papers and supporting documents may be at the booth if properly labeled as such.

4. Longitudinal studies are permitted as an acceptable continuation under the following conditions:

  • a. The study is a multi-year study testing or documenting the same variables in which time is a critical variable. (Examples: Effect of high rain or drought on soil in a given basin, return of flora and fauna in a burned area over time.)
  • b. Each consecutive year must demonstrate time-based change
  • c. The display board must be based on collective past conclusionary data and its comparison to the current year data set. No raw data from previous years may be displayed.

5. All projects must be reviewed and approved each year and forms must be completed for the new year.

6. NOTE: For competition in Regeneron ISEF, the Continuation Research Progression Project Form (7) is required for projects in the same field of study as a previous project. This form must be displayed at the project booth. Retention of all prior years’ paperwork is required and must be presented to the Regeneron ISEF SRC upon request.

Team Projects

1. Team projects compete and are judged in the category of their research at Regeneron ISEF. All team members must meet the eligibility requirements for Regeneron ISEF.

2. Teams must have no more than three members. A team with members from different geographic regions may compete at an affiliated fair of one of its members, but not at multiple fairs. However, each affiliated fair holds the authority to determine whether teams with members outside of a fair’s geographic territory are eligible to compete, understanding that if the team wins the right to attend Regeneron ISEF, all team members’ expenses must be supported by the fair.

  • a. Team membership cannot be changed during a given research year unless there are extenuating circumstances and the local SRC reviews and approves the change, including converting a team project to an individual project or vice versa. Such conversions must address rationale for the change and include a clear delineation between research preceding the change and that which will follow. A memorandum documenting this review and approval should be attached to Form 1A.
  • b. Once a project has competed in a science fair at any level, team membership cannot change and the project cannot be converted from an individual project to a team project or vice versa.
  • c. In a future research year, any project may be converted from an individual to a team project, from a team to an individual project and/or have a change in team membership.

3. Each team is encouraged to appoint a team leader to coordinate the work and act as spokesperson. However, each member of the team should be able to serve as spokesperson, be fully involved with the project, and be familiar with all aspects of the project. The final work should reflect the coordinated efforts of all team members and will be evaluated using the same judging criteria as individual projects.

4. Each team member must submit an Approval Form (1B). Team members must jointly submit the Checklist for Adult Sponsor (1), one abstract, a Student Checklist (1A), a Research Plan/Project Summary and other required forms.

5. Full names of all team members must appear on the abstract and forms.

Sources of Information for ALL Projects

1. United States Patent and Trade Office
Customer Service: 1-800-786-9199 (toll-free);
571-272-1000 (local); 571-272-9950 (TTY)
Conducting a Patent Search:

2. USPTO Resources

3. European Patent Office

4. Aquatic Nuisance Species (ANS) Task

Animal and Plant Health Inspection Service Invasive Species List

6. Invasive Species Specialist Group
The Global Invasive Species database contains invasive species information supplied by experts from around the world.

7. Invasive Species Information
Provides information for species declared invasive, noxious, prohibited, or harmful or potentially harmful.


1. Code of Federal Regulation (CFR), Title 45 (Public Welfare), Part 46-Protection of Human Subjects (45CFR46)

2. NIH tutorial, “Protecting Human Research Participants”

3. Belmont Report, April 18, 1979

4. Standards for Educational and Psychological Testing. (1999). Washington, DC: AERA, APA, NCME.

5. American Psychological Association
750 First Street, NE Washington, DC 20002-4242
phone: 202-336-5500; 800-374-2721
Information for students:
Information regarding publications:

6. Educational and Psychological Testing
Testing Office for the APA Science Directorate
phone: 202-336-6000

7. The Children’s Online Privacy Protection Act of 1998 (COPPA) (15 U.S.C. §§ 6501–6506)


Animal Care and Use

1. Laboratory Animals, Institute of Laboratory Animal Research (ILAR), Commission on Life Sciences, National Research

2. Guide for the Care and Use of Laboratory Animals, 8th Edition (2011)

3. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research (2003), Institute for Laboratory Animal Research (ILAR)
To order these ILAR publications contact:
National Academies Press
500 Fifth Street, NW
Washington, DC 20055
phone: 888-624-8373 or 202-334-3313; fax: 202-334-2451

4. Federal Animal Welfare Act (AWA)
7 U.S.C. 2131-2157
Subchapter A – Animal Welfare (Parts I, II, III)
Document is available from:
4700 River Road, Unit 84
Riverdale, MD 20737-1234
phone: 301-734-7833; fax: 301-734-4978

5. Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching (Agri-Guide)
Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC International)

6. Guidelines for the Use of Fish in Research (2014), American Fisheries Society.

7. Euthanasia Guidelines
AVMA Guidelines on Euthanasia (2020)
American Veterinary Medical Association

Alternative Research and Animal Welfare

1. The National Library of Medicine provides computer searches through MEDLINE:
Reference & Customer Services
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
888-FIND-NLM or 888-346-3656; 301-594-5983;

2. National Agriculture Library (NAL) provides reference service for materials that document a) Alternative Procedures to Animal Use and b) Animal Welfare.
Animal Welfare Information Center
National Agriculture Library
10301 Baltimore Avenue, Room 410
Beltsville, MD 20705-2351
phone: 301-504-6212, fax: 301-504-7125

3. Institute of Laboratory Animal Resources (ILAR) provides a variety of information on animal sources, housing and handling standards, and alternatives to animal use through annotated bibliographies published quarterly in ILAR Journal.
ILAR — The Keck Center of the National Academies
500 Fifth Street, NW, Keck 687
Washington, DC 20001
phone: 202-334-2590, fax: 202-334-1687

4. Quarterly bibliographies of Alternatives to the Use of Live Vertebrates in Biomedical Research and Testing may be obtained from:Specialized Information Services
2 Democracy Plaza, Suite 510
6707 Democracy Blvd., MSC 5467
Bethesda, MD 20892-5467
phone: 301-496-1131; Fax: 301- 480-3537

5. Johns Hopkins Center for Alternatives to Animal Testing (CAAT) has worked with scientists since 1981 to find new methods to replace the use of laboratory animals in experiments, reduce the number of animals tested, and refine necessary tests to eliminate pain and distress.

6. Quality Assurance Manuals (for appropriate species)
Such as:


General Lab/Chemical Safety

1. Safety in Academic Chemistry Laboratories, Volumes 1 and 2, 2003. Washington, DC: American Chemical Society.
Order from (first copy free of charge):
American Chemical Society
Publications Support Services
1155 16th Street, NW
Washington, DC 20036
phone: 202- 872-4000 or 800-227-5558

2. General
Howard Hughes Medical Institute has resources for working with cell cultures, radioactive materials and other laboratory materials.

3. Environmental Protection Agency (EPA) website for green chemistry

4. Safety and Data Sheets (SDS)
A directory of SDS sheets from Flinn Scientific Inc. that includes a ranking of hazard level and disposal methods. – A listing of numerous sites that have free downloads of SDS sheets.

NFPA (National Fire Protection Association) 704 Standard for guidance on Chemical Reactivity and Instability:

5. Pesticides
National Pesticide Information Center
Describes the various types of pesticides and the legal requirements for labelling. Provides links and phone numbers to get additional information.
Environmental Protection Agency
A database of product labels. Enter the product name or company name to view the approved label information of pesticides which are registered with the agency.

6. DEA Controlled Substances
Drug Enforcement Agency website:
Controlled Substance Schedules – a list of controlled substances:

7. Alcohol, Tobacco, Firearms, and Explosives
Alcohol and Tobacco Tax and Trade Bureau
Bureau of Alcohol, Tobacco, Firearms and Explosives

8. Radiation
Radiation Studies Information (CDC)

9. CDC Laboratory Safety Manuals

10. Occupational Safety and Health Administration
Safety and Health Topics:

11. U. S. Nuclear Regulatory Commission
Material Safety and Inspection Branch
One White Flint North
11555 Rockville Pike
Rockville, MD 20852
phone: 301-415-8200; 800-368-5642